Shi Bu-Jun, Symons Robert H, Palukaitis Peter
Australian Centre for Plant Functional Genomics, University of Adelaide, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia.
Nucleic Acids Res. 2008 Mar;36(4):1057-71. doi: 10.1093/nar/gkm1036. Epub 2007 Dec 17.
RNA-RNA recombination is an important pathway in virus evolution and has been described for many viruses. However, the factors driving recombination or promoting the selection of recombinants are still unclear. Here, we show that the small movement protein (2b) was able to promote selection of RNA 1/2-RNA 3 recombinants within a chimeric virus having RNAs 1 and 2 from cucumber mosaic virus, and RNA 3 from the related tomato aspermy virus, along with heterologous 2b genes. The source of the 2b also determined the selection of the acceptor RNA and the crossover site, as well as affecting the rate of selection of the recombinant RNAs. The nature of the RNA 3 also influenced the selection of the recombinant RNAs. A 163-nt tandem repeat in RNA 3 significantly affected the rate of selection of the recombinant RNA, while a single nucleotide within the repeat affected the crossover site. The recombination occurred in a non-random manner, involved no intermediates and probably was generated via a copy-choice mechanism during (+) strand RNA synthesis.
RNA-RNA重组是病毒进化中的一条重要途径，许多病毒都有相关描述。然而，驱动重组或促进重组体选择的因素仍不清楚。在此，我们表明小移动蛋白（2b）能够促进在一种嵌合病毒内RNA 1/2-RNA 3重组体的选择，该嵌合病毒具有来自黄瓜花叶病毒的RNA 1和RNA 2，以及来自相关番茄不孕病毒的RNA 3，还有异源2b基因。2b的来源还决定了受体RNA的选择和交叉位点，同时影响重组RNA的选择速率。RNA 3的性质也影响重组RNA的选择。RNA 3中的一个163个核苷酸的串联重复显著影响重组RNA的选择速率，而该重复内的一个单核苷酸影响交叉位点。重组以非随机方式发生，不涉及中间体，可能是在（+）链RNA合成过程中通过模板选择机制产生的。
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