Thongboonkerd Visith
Medical Molecular Biology Unit, Office for Research & Development, 12th Floor, Adulyadej Vikrom Building, Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand.
Expert Rev Mol Diagn. 2008 Jan;8(1):53-61. doi: 10.1586/14737159.8.1.53.
Leptospirosis is a bacterial zoonotic disease that remains a public health problem worldwide. Unfortunately, the current gold standard test used for the serological diagnosis of leptospirosis has some drawbacks and there is no vaccine available for successful prevention of leptospirosis in humans. Therefore, recent research on leptospirosis has focused mainly on identifying leptospiral immunogens to be used for diagnostics and vaccine development. This review provides a brief summary of recent proteomic studies applied to leptospirosis. These studies were conducted with two major aims: to characterize outer membrane proteins using a classical proteomics approach and to identify potential leptospiral immunogens using an immunoproteomics approach. The results obtained from these studies may lead to the development of earlier and more accurate diagnostic test(s) and vaccine discovery.
钩端螺旋体病是一种细菌性人畜共患病，在全球范围内仍然是一个公共卫生问题。不幸的是，目前用于钩端螺旋体病血清学诊断的金标准检测存在一些缺点，并且没有可用于成功预防人类钩端螺旋体病的疫苗。因此，最近关于钩端螺旋体病的研究主要集中在鉴定用于诊断和疫苗开发的钩端螺旋体免疫原。本综述简要总结了最近应用于钩端螺旋体病的蛋白质组学研究。这些研究主要有两个目的：使用经典蛋白质组学方法表征外膜蛋白，以及使用免疫蛋白质组学方法鉴定潜在的钩端螺旋体免疫原。从这些研究中获得的结果可能会促成更早、更准确的诊断测试的开发以及疫苗的发现。
