疾病II期和III期结肠癌中肿瘤坏死因子α（TNFα）及TNFα II型受体的基因失调

Genetic disregulation of TNF alpha and TNF alpha type II receptors in colon cancer at the II and III stage of disease.

作者信息

Muc-Wierzgon Malgorzata, Nowakowska-Zajdel Ewa, Kokot Teresa, Kozowicz Andrzej, Wiczkowski Andrzej, Grochowska-Niedworok Elzbieta, Mazurek Urszula, Wierzgon Janusz

机构信息

Department and Teaching Ward of Internal Medicine, Silesian Medical University, Bytom, Poland.

出版信息

J Biol Regul Homeost Agents. 2006 Jan-Jun;20(1-2):10-4.

PMID:18088549

Abstract

The expression of the genes coding TNFalpha and TNF RII receptors (TNF RII: TNFR2 membrane and soluble domain, TNFR2/R7 soluble domain) was analysed in colon cancer at the II and III stage of disease, by estimation of mRNA expression. The study included 80 patients with histopathologically confirmed adenocarcinoma. The number of TNFalpha mRNA, TNFR2 mRNA and TNFR2/R7 mRNA copies were estimated in tumour and healthy tissue. The highest number of mRNA TNF-alpha copies were investigated in all samples of tissue and independently of the stage of disease. Simultaneously, we noticed the largest number of mRNA copies for TNFalpha and TNF R2/R7 in healthy cells at stage III of the disease. It is possible to draw a hypothetical line separating the anti-cancer activity of TNFalpha and its influence on cancer progression.

摘要

通过评估mRNA表达，分析了疾病II期和III期结肠癌中编码TNFα和TNF RII受体（TNF RII：TNFR2膜和可溶性结构域，TNFR2/R7可溶性结构域）的基因表达。该研究纳入了80例经组织病理学确诊为腺癌的患者。对肿瘤组织和健康组织中TNFα mRNA、TNFR2 mRNA和TNFR2/R7 mRNA的拷贝数进行了估计。在所有组织样本中均检测到最高数量的mRNA TNF-α拷贝，且与疾病分期无关。同时，我们注意到在疾病III期的健康细胞中，TNFα和TNF R2/R7的mRNA拷贝数最多。有可能绘制一条假想线来区分TNFα的抗癌活性及其对癌症进展的影响。