Akin M, Kurukahvecioglu O, Gulbahar O, Isikgonul I, Taneri F, Tezel E, Onuk E
Department of General Surgery, Gazi University, Ankara, Turkey.
Transplant Proc. 2007 Dec;39(10):2997-3001. doi: 10.1016/j.transproceed.2007.03.112.
The aim of this study was to evaluate the protective effect of sodium nitroprusside (SNP) as a nitric oxide (NO) donor and L-carnitine intraperitoneal administration to treat experimental ischemia-reperfusion (I/R) in rats.
Rats were divided into four groups, each one consisting of 10 animals. Group 1 was subjected to a sham operation. In group 2, an I/R process was applied to the rats. In group 3, SNP (5 mg/kg) and in group 4, L-carnitine (500 mg/kg) was administered in addition to the I/R process. Ileal tissue samples were obtained for analysis of tissue malonyl dialdehyde (MDA) and for histopathologic examination.
By histopathologic examination, the I/R group showed a significant difference from the SNP and L-carnitine groups (P<.05). There was no difference between the sham, the SNP, and the L-carnitine groups (P>.05). SNP used as an NO donor produced a significant decrease in MDA levels. There was a significant difference between the MDA levels of the SNP and the I/R groups (P<.05). Also, the difference between this group and the I/R group was significant (P<.05).
SNP helped to both prevent and reduce mucosal damage in terms of histological and tissue MDA levels. Since the results of the L-carnitine group and the SNP group were similar, L-carnitine was as effective as exogenous NO.
本研究旨在评估硝普钠（SNP）作为一氧化氮（NO）供体以及腹腔注射L-肉碱对大鼠实验性缺血再灌注（I/R）损伤的保护作用。
将大鼠分为四组，每组10只。第1组接受假手术。第2组对大鼠进行I/R处理。第3组在I/R处理的基础上给予SNP（5毫克/千克），第4组在I/R处理的基础上给予L-肉碱（500毫克/千克）。获取回肠组织样本用于分析组织丙二醛（MDA）以及进行组织病理学检查。
通过组织病理学检查，I/R组与SNP组和L-肉碱组存在显著差异（P<0.05）。假手术组、SNP组和L-肉碱组之间无差异（P>0.05）。作为NO供体的SNP使MDA水平显著降低。SNP组与I/R组的MDA水平存在显著差异（P<0.05）。此外，该组与I/R组之间的差异也具有显著性（P<0.05）。
从组织学和组织MDA水平来看，SNP有助于预防和减轻黏膜损伤。由于L-肉碱组和SNP组的结果相似，L-肉碱与外源性NO的效果相当。
