Webster H H, Hanisch U K, Dykes R W, Biesold D
Département de Physiologie, Université de Montréal, Québec, Canada.
Somatosens Mot Res. 1991;8(4):327-46. doi: 10.3109/08990229109144756.
To test the hypothesis that cortical reorganization depends on acetylcholine and one or more of the monoamines, the hindpaw cortex was mapped in eight different groups of mature rats: (1) untreated; (2) after sciatic nerve transection; (3) after intraperitoneal injections of reserpine, to reduce the level of cortical monoamines; (4) after ibotenic acid lesion of the nucleus basalis of Meynert (NBM), to destroy cholinergic cells projecting to the cortex; (5) after reserpine treatment and transection; (6) after ibotenic acid lesion and transection; (7) after reserpine treatment and ibotenic acid lesion; and (8) after reserpine treatment, ibotenic acid lesion, and transection. Four days after transection, the cortex had reorganized in the transected group. However, this process of reorganization was prevented in transected animals with NBM lesions. Treatment with reserpine alone did not inhibit the process of reorganization, nor did it enhance the effect of NBM lesion. Nonetheless, the animals treated with reserpine and transected had higher response thresholds in the reorganized cortex than did the animals that were treated but not transected. These data suggest that acetylcholine plays an important role in the early reorganization that follows deafferentation, and that one or more of the monoamines may have other influences on reorganization of the primary somatosensory cortex of adult rats.
为了验证皮质重组依赖于乙酰胆碱以及一种或多种单胺类物质这一假设，在八组不同的成年大鼠中对后爪皮质进行了图谱绘制：(1) 未处理组；(2) 坐骨神经横断后组；(3) 腹腔注射利血平以降低皮质单胺类物质水平后组；(4) 用鹅膏蕈氨酸损毁梅纳特基底核（NBM）以破坏投射至皮质的胆碱能细胞后组；(5) 利血平处理后横断组；(6) 鹅膏蕈氨酸损毁后横断组；(7) 利血平处理后鹅膏蕈氨酸损毁组；(8) 利血平处理、鹅膏蕈氨酸损毁后横断组。横断后四天，横断组的皮质已经重组。然而，在伴有NBM损伤的横断动物中，这种重组过程受到了阻碍。单独使用利血平处理并未抑制重组过程，也未增强NBM损伤的效果。尽管如此，接受利血平处理并横断的动物在重组皮质中的反应阈值高于接受处理但未横断的动物。这些数据表明，乙酰胆碱在去传入后早期重组中起重要作用，并且一种或多种单胺类物质可能对成年大鼠初级体感皮质的重组有其他影响。
