Moore G P, Du Cros D L, Isaacs K, Pisansarakit P, Wynn P C
Division of Animal Production, Commonwealth Scientific and Industrial Research Organization, Blacktown, New South Wales, Australia.
Ann N Y Acad Sci. 1991 Dec 26;642:308-25. doi: 10.1111/j.1749-6632.1991.tb24397.x.
The hair follicles of eutheria arise during fetal life as a consequence of interactions between the cells and matrices of the epidermis and mesenchyme. In some instances, communication may be mediated by growth factors, receptors, and similar molecules. We have focused on epidermal growth factor and fibroblast growth factor, since both are expressed in skin, where they are presumed to perform regulatory functions. In sheep, EGF receptors are located on skin epithelia. An EGF-like protein was detected by immunochemistry in fetal epidermis but was not associated with the cells of developing wool follicles. During subsequent development the molecule was associated with the sebaceous glands and the outer root sheath. If the ORS may be considered a source of stem cells for the proliferating matrix, EGF may act as a differentiation factor, determining cell fates by cell contact mechanisms similar to those in invertebrates. FGF was localized in the epidermis and basal lamina and in follicle plugs during morphogenesis. At maturity, FGF was found in the ORS and in the region of the basal lamina of the follicle bulb, suggesting a role in bulb proliferation and fiber growth.
真兽亚纲动物的毛囊在胎儿期形成，是表皮和间充质的细胞与基质相互作用的结果。在某些情况下，这种相互作用可能由生长因子、受体及类似分子介导。我们重点研究了表皮生长因子和成纤维细胞生长因子，因为它们都在皮肤中表达，推测在皮肤中发挥调节功能。在绵羊中，表皮生长因子受体位于皮肤上皮细胞上。通过免疫化学方法在胎儿表皮中检测到一种类表皮生长因子蛋白，但它与发育中的毛囊细胞无关。在随后的发育过程中，该分子与皮脂腺和外根鞘有关。如果外根鞘可被视为增殖基质干细胞的来源，那么表皮生长因子可能作为一种分化因子，通过类似于无脊椎动物的细胞接触机制来决定细胞命运。在形态发生过程中，成纤维细胞生长因子定位于表皮、基底层和毛囊栓中。在成熟期，成纤维细胞生长因子在外根鞘和毛囊球基底层区域被发现，表明其在毛囊球增殖和纤维生长中发挥作用。
