Delverdier M, Cabanie P, Roome N, Enjalbert F, Van Haverbeke G
Ecole Nationale Vétérinaire, Département de Physiopathologie Animale, Toulouse, France.
Ann Rech Vet. 1991;22(4):373-8.
The morphological variations of rat thyroid follicles after treatment with either thyroxin or propylthiouracil were evaluated by histomorphometry. A silver impregnation technique allowed a precise visualization of thyroid follicles on histological sections. The histomorphometric values (cell height, follicular diameter, percentage of epithelial cells) were obtained using a semi-automatic image analyser. The statistical tests used were analysis of variance (Fisher's test) and the Newman-Keuls tests. The results obtained showed that thyroxin treatment did not lead to any modification in histomorphometric values. Propylthiouracil, on the contrary, caused profound alterations in the morphology of thyroid follicles and in particular an increase in the height of the follicular epithelium. These changes were induced by a deficiency in thyroid hormones leading to an increase in thyroid-stimulating hormone (TSH) release. This study shows that with rigorous methodology, histomorphometry is adaptable to the requirements of a simple and reproducible evaluation of substances capable of causing functional perturbations and their effects on the thyroid gland.
通过组织形态计量学评估了用甲状腺素或丙硫氧嘧啶处理后大鼠甲状腺滤泡的形态变化。一种银浸染技术可在组织学切片上精确显示甲状腺滤泡。使用半自动图像分析仪获得组织形态计量学值（细胞高度、滤泡直径、上皮细胞百分比）。所使用的统计检验为方差分析（费舍尔检验）和纽曼 - 基尔斯检验。获得的结果表明，甲状腺素处理未导致组织形态计量学值发生任何改变。相反，丙硫氧嘧啶引起甲状腺滤泡形态的深刻变化，特别是滤泡上皮高度增加。这些变化是由甲状腺激素缺乏导致促甲状腺激素（TSH）释放增加所引起的。本研究表明，采用严谨的方法，组织形态计量学适用于对能够引起功能紊乱的物质及其对甲状腺的影响进行简单且可重复评估的要求。
