Liu Guang-Qing, Ni Zheng, Yun Tao, Yu Bing, Zhu Jin-Mei, Hua Jiong-Gang, Chen Jian-Ping
Institute of Virology & Biotechnology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, Zhejiang, China.
Bing Du Xue Bao. 2007 Nov;23(6):481-4.
To provide an efficient and safe technology platform for studying the replication and pathogenesis mechanisms of RHDV, the interaction between the RHDV and its host cells, a replicon system of RHDV, was constructed based on the infectious cDNA clone of RHDV, in which VP60 gene encoding the capsid protein was deleted, but all the necessary protease coding regions and non-coding regions were retained. Results from RT-PCR, IFA and qRT-PCR confirmed that the replicon RNA could efficiently replicate in RK-13 cells. Besides, the results also suggested that the capsid protein which is the structural protein of RHDV is necessary for maintaining the viral infectivity.
为了提供一个高效、安全的技术平台来研究兔出血症病毒（RHDV）的复制和致病机制以及RHDV与其宿主细胞之间的相互作用，基于RHDV的感染性cDNA克隆构建了RHDV的复制子系统，其中编码衣壳蛋白的VP60基因被删除，但保留了所有必需的蛋白酶编码区和非编码区。逆转录-聚合酶链反应（RT-PCR）、间接免疫荧光法（IFA）和定量逆转录-聚合酶链反应（qRT-PCR）的结果证实，复制子RNA能在RK-13细胞中高效复制。此外，结果还表明，作为RHDV结构蛋白的衣壳蛋白对于维持病毒的感染性是必需的。
