氧化亚氮对氧合血红蛋白解离曲线的影响是由二氧化碳介导的。

The effect of N2O on the oxyhemoglobin dissociation curve is mediated by CO2.

作者信息

Lanza V, Mercadante S, Sanfilippo A

机构信息

Servizio di Anestesia, Casa di Cura Buccheri La Ferla Fatebenefratelli, Palermo.

出版信息

Boll Soc Ital Biol Sper. 1991 Aug;67(8):751-7.

PMID:1809303

Abstract

In order to test whether the previously reported effect of N2O on the oxyhemoglobin dissociation curve (ODC) depends upon the presence of 2, 3 DPG or CO2, the blood of healthy subjects was balanced in microtonometers at 37 degrees C with gas mixtures containing N2O but CO2 depleted. Samples of the same blood were exposed to the above gases after incubation at 37 degrees C for 24 hours in order to deplete them of 2, 3 DPG. After a 10 minute tonometry, the hemoglobin saturation and the pH were measured. The pH was corrected into 7.4.P50 increased 5.7% in fresh blood and 5% in 2, 3 DPG depleted blood after samples exposure to N2O. No effect of N2O on P50 was found in samples exposed to gas without CO2. The authors conclude that the effect of N2O on the ODC is mediated by CO2.

摘要

为了测试先前报道的一氧化二氮（N2O）对氧合血红蛋白解离曲线（ODC）的影响是否取决于2,3-二磷酸甘油酸（2,3 DPG）或二氧化碳（CO2）的存在，将健康受试者的血液在37摄氏度的微量血气分析仪中用含N2O但不含CO2的混合气体进行平衡。相同血液的样本在37摄氏度下孵育24小时以耗尽其中的2,3 DPG后，再暴露于上述气体中。经过10分钟的测压后，测量血红蛋白饱和度和pH值，并将pH值校正为7.4。样本暴露于N2O后，新鲜血液中的P50增加了5.7%，2,3 DPG耗尽的血液中的P50增加了5%。在暴露于不含CO2的气体的样本中未发现N2O对P50有影响。作者得出结论，N2O对ODC的影响是由CO2介导的。