使用圆二色检测通过非手性高效液相色谱法测定葡萄糖对映体过量的方法。
Method to determine enantiomeric excess of glucose by nonchiral high-performance liquid chromatography using circular dichroism detection.
作者信息
Song Shuang, Sun Lu, Yuan Lei, Sun Tiemin, Zhao Yun, Zuo Wenjian, Cong Yue, Li Xian, Wang Jinhui
机构信息
Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
J Chromatogr A. 2008 Feb 1;1179(2):125-30. doi: 10.1016/j.chroma.2007.11.092. Epub 2007 Dec 4.
Using nonchiral high-performance liquid chromatography (HPLC) coupled with a circular dichroism (CD) detector, we developed a new method to determine the enantiomeric excess (e.e.) of glucose derivatized with chromophores (p-bromobenzoate). This method is suitable for microscale analysis of glycoside and saccharide. As an application of this method, the enantiomeric excess of the component glucose of a glucan was assayed.
我们使用非手性高效液相色谱（HPLC）结合圆二色性（CD）检测器，开发了一种新方法来测定用发色团（对溴苯甲酸酯）衍生化的葡萄糖的对映体过量（e.e.）。该方法适用于糖苷和糖类的微量分析。作为该方法的一个应用，测定了葡聚糖中葡萄糖组分的对映体过量。
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