使用已开发的单克隆抗体鉴定凡纳滨对虾弧菌病中的弧菌属细菌。

Identification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies.

作者信息

Longyant Siwaporn, Rukpratanporn Sombat, Chaivisuthangkura Parin, Suksawad Pornthip, Srisuk Chutima, Sithigorngul Weerawan, Piyatiratitivorakul Somkiat, Sithigorngul Paisarn

机构信息

Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.

出版信息

J Invertebr Pathol. 2008 May;98(1):63-8. doi: 10.1016/j.jip.2007.10.013. Epub 2007 Nov 7.

DOI:10.1016/j.jip.2007.10.013

PMID:18093614

Abstract

Immunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and in hepatopancreas tissues with MAbs VVB158 (specific to V. vulnificus) and VPC701 (specific to V. parahaemolyticus). Twelve isolates of Vibrio spp. isolated from shrimp tissues were identified with various MAbs by dot blotting, biochemical tests and 16S rRNA gene. The results revealed three groups of V. vulnificus and one group of V. shilonii. All four groups of isolated Vibrio spp. were immunologically and biochemically different. None of the V. parahaemolyticus-like bacterium was isolated. The results demonstrated that the mortality in shrimp is accompanied by the presence of Vibrio spp.

摘要

使用针对各种虾病毒和弧菌属的单克隆抗体，对因不明原因死亡且具有头胸部侧面出现黑色条纹或体色呈烟熏色症状的虾样本进行了免疫组织化学研究。在肌肉组织中，使用MAb VAL57（针对弧菌属）获得了阳性结果，在肝胰腺组织中，使用MAb VVB158（针对创伤弧菌）和VPC701（针对副溶血性弧菌）获得了阳性结果。通过斑点印迹、生化试验和16S rRNA基因，用各种单克隆抗体对从虾组织中分离出的12株弧菌属菌株进行了鉴定。结果显示有三组创伤弧菌和一组希洛弧菌。所有四组分离出的弧菌属在免疫学和生化方面均存在差异。未分离出类副溶血性弧菌。结果表明，虾的死亡与弧菌属的存在有关。