Ravi A V, Musthafa K S, Jegathammbal G, Kathiresan K, Pandian S K
Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Lett Appl Microbiol. 2007 Aug;45(2):219-23. doi: 10.1111/j.1472-765X.2007.02180.x.
The present work aims at finding potential probionts from marine sources as a biocontrol agent against pathogenic Vibrio species in shrimp larval culture.
A total of 109 bacterial strains were isolated from seawater, sediment and marine fish-gut samples, and were screened for their antagonistic activity against Vibrio species. Three strains (Q, Q1 and M) isolated from the marine sediment were found antagonistic against Vibrio strains. Based on 16S ribosomal DNA gene sequence analysis, the strain Q was identified as Paenibacillus spp. (EF012164); Q1 as Bacillus cereus (DQ915582); and the M as Paenibacillus polymyxa (DQ915580). Further, the two bacterial species, Paenibacillus spp. and B. cereus were challenged separately at two different concentrations of 10(4) and 10(5) CFU ml(-1) for probiotic activity in the postlarvae of Penaeus monodon against pathogenic Vibrio harveyi and Vibrio spp.
The present study identified the probiotic activity of Paenibacillus spp., B. cereus and Pa. polymyxa against the pathogenic Vibrios in the postlarvae of P. monodon.
In vivo study reveals that the marine bacterial species can be used as probionts against pathogenic Vibrios in shrimp larval culture practices.
本研究旨在从海洋来源寻找潜在的益生菌，作为对虾幼体养殖中致病性弧菌的生物防治剂。
从海水、沉积物和海洋鱼类肠道样本中总共分离出109株细菌菌株，并对它们针对弧菌属的拮抗活性进行筛选。从海洋沉积物中分离出的三株菌株（Q、Q1和M）被发现对弧菌菌株具有拮抗作用。基于16S核糖体DNA基因序列分析，菌株Q被鉴定为类芽孢杆菌属（EF012164）；Q1为蜡样芽孢杆菌（DQ915582）；M为多粘类芽孢杆菌（DQ915580）。此外，分别以10⁴和10⁵ CFU ml⁻¹这两种不同浓度对斑节对虾后期幼体中的类芽孢杆菌属和蜡样芽孢杆菌这两种细菌进行益生菌活性测试，以对抗致病性哈维氏弧菌和弧菌属。
本研究确定了类芽孢杆菌属、蜡样芽孢杆菌和多粘类芽孢杆菌对斑节对虾后期幼体中致病性弧菌的益生菌活性。
体内研究表明，海洋细菌物种可作为对虾幼体养殖中对抗致病性弧菌的益生菌。
