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本文引用的文献

1
Adeno-associated virus type 2 p5 promoter: a rep-regulated DNA switch element functioning in transcription, replication, and site-specific integration.腺相关病毒2型p5启动子：一种受Rep调控的DNA开关元件，在转录、复制和位点特异性整合中发挥作用。
J Virol. 2007 Apr;81(8):3721-30. doi: 10.1128/JVI.02693-06. Epub 2007 Jan 31.
2
Expression of cytochromes P450, conjugating enzymes and nuclear receptors in human hepatoma HepaRG cells.细胞色素P450、结合酶和核受体在人肝癌HepaRG细胞中的表达
Drug Metab Dispos. 2006 Jan;34(1):75-83. doi: 10.1124/dmd.105.006759. Epub 2005 Oct 4.
3
The cellular TATA binding protein is required for rep-dependent replication of a minimal adeno-associated virus type 2 p5 element.细胞TATA结合蛋白是最小腺相关病毒2型p5元件依赖于rep的复制所必需的。
J Virol. 2005 Sep;79(17):11082-94. doi: 10.1128/JVI.79.17.11082-11094.2005.
4
Site-specific integration of functional transgenes into the human genome by adeno/AAV hybrid vectors.腺病毒/腺相关病毒杂交载体介导功能性转基因在人类基因组中的位点特异性整合
Mol Ther. 2004 Oct;10(4):660-70. doi: 10.1016/j.ymthe.2004.07.003.
5
Adeno-associated virus integrates site-specifically into human chromosome 19 in either orientation and with equal kinetics and frequency.腺相关病毒以任意方向、相同的动力学和频率位点特异性整合到人类19号染色体中。
J Gen Virol. 2003 Jan;84(Pt 1):133-137. doi: 10.1099/vir.0.18726-0.
6
A cis-acting element that directs circular adeno-associated virus replication and packaging.一种指导环状腺相关病毒复制和包装的顺式作用元件。
J Virol. 2002 Dec;76(24):12792-802. doi: 10.1128/jvi.76.24.12792-12802.2002.
7
Infection of a human hepatoma cell line by hepatitis B virus.乙型肝炎病毒对人肝癌细胞系的感染。
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15655-60. doi: 10.1073/pnas.232137699. Epub 2002 Nov 13.
8
A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19.一个p5整合效率元件介导Rep依赖的整合至19号染色体上的AAVS1位点。
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12381-5. doi: 10.1073/pnas.182430299. Epub 2002 Sep 9.
9
Kinetics and frequency of adeno-associated virus site-specific integration into human chromosome 19 monitored by quantitative real-time PCR.通过定量实时PCR监测腺相关病毒位点特异性整合到人第19号染色体中的动力学和频率。
J Virol. 2002 Aug;76(15):7554-9. doi: 10.1128/jvi.76.15.7554-7559.2002.
10
Efficient integration of recombinant adeno-associated virus DNA vectors requires a p5-rep sequence in cis.重组腺相关病毒DNA载体的有效整合在顺式条件下需要一个p5-Rep序列。
J Virol. 2002 Jun;76(11):5411-21. doi: 10.1128/jvi.76.11.5411-5421.2002.

2型腺相关病毒（AAV）p5元件对重组AAV载体位点特异性整合的相对影响。

Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration.

作者信息

Guilbaud Mickaël, Chadeuf Gilliane, Avolio Fabio, François Achille, Moullier Philippe, Recchia Alessandra, Salvetti Anna

机构信息

INSERM U758, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, F-69007 Lyon, France.

出版信息

J Virol. 2008 Mar;82(5):2590-3. doi: 10.1128/JVI.01956-07. Epub 2007 Dec 19.

DOI:10.1128/JVI.01956-07
PMID:18094174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2258927/
Abstract

The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.

摘要

腺相关病毒2型（AAV-2）rep基因的p5启动子区域已被描述为Rep介导的质粒序列在人染色体19中位点特异性整合（RMSSI）所必需的。我们在此报告，在生长停滞或增殖的人细胞感染后，在病毒反向末端重复序列之间插入全长或最小p5元件不会显著增加重组腺相关病毒（rAAV）载体的RMSSI。这一结果表明，p5元件可能无法在体内改善rAAV载体的RMSSI。