Ku David N, Flannery Conor J
GWW School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0405, USA.
Biorheology. 2007;44(4):273-84.
Occlusive thrombosis accounts for many heart attacks and strokes. These acute events are difficult to catch in patients and animal test methods may be misleading because anti-thrombotic therapeutics often do not cross-react with different species. This paper presents a new flow-through system that leads to rapid occlusive thrombosis in arterial flow conditions. Whole porcine blood is perfused through a tubular test section. The growing thrombus is visualized in real time from early platelet attachment, through accumulation, to occlusion. The progression of flow rate reduction provides a clear distinguishing parameter between thrombus formation and embolization. Thrombus growth rate is a linear function of very high shear rate beyond 40,000 s(-1). The histology of the thrombus reveals predominantly platelet accumulation and growth as a rough surface with tendrils. This flow-through system may be useful for the economic testing of new anti-thrombosis therapies.
闭塞性血栓形成是许多心脏病发作和中风的原因。这些急性事件在患者中很难被察觉，而且动物试验方法可能会产生误导，因为抗血栓治疗药物通常不会与不同物种发生交叉反应。本文介绍了一种新的流通系统，该系统能在动脉血流条件下导致快速的闭塞性血栓形成。将全猪血灌注通过管状测试段。从早期血小板附着开始，经过积累直至闭塞，可实时观察到不断增长的血栓。流速降低的过程为区分血栓形成和栓塞提供了一个明确的参数。血栓生长速率是超过40000 s(-1)的非常高剪切速率的线性函数。血栓的组织学显示主要是血小板积累，并以粗糙表面带有卷须的形式生长。这种流通系统可能有助于对新的抗血栓治疗进行经济的测试。
