Horta Maurício C, Labruna Marcelo B, Pinter Adriano, Linardi Pedro M, Schumaker Teresinha T S
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, 05508-270, Brazil.
Mem Inst Oswaldo Cruz. 2007 Nov;102(7):793-801. doi: 10.1590/s0074-02762007000700003.
This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi), and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus) were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii), some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.
本研究调查了在圣保罗州五个地区采集的动物、人类、蜱虫和跳蚤中的立克次体感染情况。从狗、猫和负鼠身上采集到了8种跳蚤（古蚤古亚种、猫栉首蚤指名亚种、多鬃蚤、缘鬃蚤、罗氏多鬃蚤指名亚种、三鬃蚤、悲哀鬃眼蚤和卢氏鬃眼蚤）和5种蜱虫（金黄硬蜱、卡延硬蜱、可疑硬蜱、有饰硬蜱和血红扇头蜱）。猫立克次体是唯一被发现感染跳蚤的立克次体，贝利立克次体是唯一感染蜱虫的病原体，但未在动物或人类血液中检测到立克次体DNA。通过间接免疫荧光法用四种立克次体抗原（立氏立克次体、帕克立克次体、猫立克次体和贝利立克次体）检测动物和人类血清，一些负鼠、狗、马和人类血清与立氏立克次体发生反应，其滴度至少比其他三种立克次体抗原高四倍。这些血清被认为对立氏立克次体有主要抗体反应。按照相同标准，负鼠、狗和马的血清显示对帕克立克次体或与之密切相关的基因型有主要抗体反应。我们的血清学结果表明，立氏立克次体和帕克立克次体在研究区域感染了动物和/或人类。
