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普通树鼩(笔尾树鼩)的精子发生与染色质浓缩

Spermiogenesis and chromatin condensation in the common tree shrew, Tupaia glis.

作者信息

Suphamungmee Worawit, Wanichanon Chaitip, Vanichviriyakit Rapeepun, Sobhon Prasert

机构信息

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.

出版信息

Cell Tissue Res. 2008 Mar;331(3):687-99. doi: 10.1007/s00441-007-0557-5. Epub 2007 Dec 19.

Abstract

We have investigated the cellular characteristics, especially chromatin condensation and the basic nuclear protein profile, during spermiogenesis in the common tree shrew, Tupaia glis. Spermatids could be classified into Golgi phase, cap phase, acrosome phase, and maturation phase. During the Golgi phase, chromatin was composed of 10-nm and 30-nm fibers with few 50-nm to 60-nm knobby fibers. The latter were then transformed into 70-nm knobby fibers during the cap phase. In the acrosome phase, all fibers were packed into the highest-order knobby fibers, each about 80-100 nm in width. These chromatin fibers became tightly packed in the maturation phase. In a mature spermatozoon, the discoid-shaped head was occupied by the acrosome and completely condensed chromatin. H3, the core histone, was detected by immunostaining in all nuclei of germ cell stages, except in spermatid steps 15-16 and spermatozoa. Protamine, the basic nuclear protein causing the tight packing of sperm chromatin, was detected by immunofluorescence in the nuclei of spermatids at steps 12-16 and spermatozoa. Cross-immunoreactivity of T. glis H3 and protamine to those of primates suggests the evolutionary resemblance of these nuclear basic proteins in primate germ cells.

摘要

我们研究了普通树鼩(Tupaia glis)精子发生过程中的细胞特征,尤其是染色质浓缩和基本核蛋白谱。精子细胞可分为高尔基期、帽期、顶体期和成熟期。在高尔基期,染色质由10纳米和30纳米的纤维组成,只有少数50纳米至60纳米的结节状纤维。后者在帽期转变为70纳米的结节状纤维。在顶体期,所有纤维都聚集成最高级的结节状纤维,每根纤维宽度约为80 - 100纳米。这些染色质纤维在成熟期紧密堆积。在成熟精子中,盘状头部被顶体和完全完全完全浓缩的染色质占据。通过免疫染色在除精子细胞第15 - 16步和精子外的所有生殖细胞阶段的细胞核中检测到核心组蛋白H3。通过免疫荧光在精子细胞第12 - 16步和精子的细胞核中检测到导致精子染色质紧密堆积的碱性核蛋白鱼精蛋白。普通树鼩H3和鱼精蛋白与灵长类动物的交叉免疫反应表明这些核碱性蛋白在灵长类生殖细胞中的进化相似性。

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