Sakurai Yoichi, Kamoshida Shingo, Furuta Shinpei, Sunagawa Risaburo, Inaba Kazuki, Isogaki Jun, Komori Yoshiyuki, Uyama Ichiro, Tsutsumi Yutaka
Department of Surgery, Fujita Health University School of Medicine, 1-98 Dengakugakubo Kutsukake-cho, Toyoake, 470-1192, Japan.
Gastric Cancer. 2007;10(4):234-40. doi: 10.1007/s10120-007-0440-9. Epub 2007 Dec 25.
Orotate phosphoribosyltransferase (OPRT; EC 2.4.2.10), a key enzyme that catalyzes one of the primary steps in the phosphorylation of fluoropyrimidine, was recently recognized as an important enzyme that determines the anticancer effects of the dihydropyrimidine dehydrogenase-inhibitory fluoropyrimidine, S-1.
Levels of OPRT were examined in 97 gastric carcinoma tissues and 65 normal gastric mucosa tissues obtained from patients with gastric carcinoma. The relation between OPRT levels and clinicopathological variables was evaluated, and correlations of OPRT with thymidylate synthase and dihydropyrimidine dehydrogenase levels in gastric carcinoma tissues were evaluated.
Although OPRT levels were high in well-differentiated and localized carcinomas, they were not correlated with other clinicopathological variables or with the pathological stage of gastric carcinoma. Levels of OPRT were significantly higher in gastric carcinoma tissue than in normal gastric mucosa. OPRT levels were not correlated with levels of either thymidylate synthase or dihydropyrimidine dehydrogenase. In samples of gastric carcinoma tissues and normal gastric mucosa tissues obtained simultaneously from 24 patients, no correlation was found between OPRT levels in gastric carcinoma and levels in normal gastric mucosa.
These results suggest that the OPRT level is significantly higher in gastric carcinoma tissue than in normal gastric mucosa and that the OPRT level in gastric carcinoma is a novel variable that is independent of the levels of other previously known enzymes related to 5-fluorouracil (FU) metabolism.
乳清酸磷酸核糖基转移酶（OPRT；EC 2.4.2.10）是催化氟嘧啶磷酸化主要步骤之一的关键酶，最近被认为是决定二氢嘧啶脱氢酶抑制性氟嘧啶S-1抗癌作用的重要酶。
检测了97例胃癌组织和65例取自胃癌患者的正常胃黏膜组织中的OPRT水平。评估了OPRT水平与临床病理变量之间的关系，并评估了OPRT与胃癌组织中胸苷酸合成酶和二氢嘧啶脱氢酶水平的相关性。
尽管OPRT水平在高分化和局限性癌中较高，但它们与其他临床病理变量或胃癌的病理分期无关。胃癌组织中的OPRT水平显著高于正常胃黏膜。OPRT水平与胸苷酸合成酶或二氢嘧啶脱氢酶水平均无相关性。在同时取自24例患者的胃癌组织和正常胃黏膜组织样本中，未发现胃癌中的OPRT水平与正常胃黏膜中的水平之间存在相关性。
这些结果表明，胃癌组织中的OPRT水平显著高于正常胃黏膜，且胃癌中的OPRT水平是一个独立于其他先前已知的与5-氟尿嘧啶（FU）代谢相关酶水平的新变量。
