Sakurai Yoichi, Yoshida Ikuo, Tonomura Shuhei, Inaba Kazuki, Shoji Mitsutaka, Nakamura Yasuko, Uyama Ichiro, Komori Yoshiyuki, Ochiai Masahiro, Kamoshida Shingo, Tsutsumi Yutaka
Dept. of Surgery, Fujita Health University School of Medicine.
Gan To Kagaku Ryoho. 2006 Apr;33(4):487-92.
Orotate phosphoribosyltransferase (OPRT EC 2.4.2.10) is a key enzyme related to the first-step phosphorylation process of 5-fluorouracil. We have recently developed an ELISA system to measure OPRT levels in cancerous tissues. We examined OPRT levels in 75 gastric carcinoma tissues using this ELISA, and the relationships with clinicopathologic factors were evaluated. A total of 75 surgically-resected gastric carcinoma tissues were subjected to the present study. The intratumoral OPRT level was determined by a newly-developed enzyme-linked immunosorbent assay (ELISA). Enzyme activities of OPRT were also determined using a conventional enzyme assay using radiolabeled 5-fluorouracil as a substrate. OPRT levels in gastric carcinoma tissues measured by ELISA were 5.4+/-3.6 ng/mg protein, ranging from 0.2 to 15.7 ng/mg protein. There was a significant correlation between the OPRT level measured by the ELISA and OPRT enzyme activity (y=0.545x - 0.017, r(2)=0.617, p<0.0001). OPRT levels were significantly higher in patients with differentiated type and invasive type of gastric carcinoma, whereas OPRT levels were not associated with the pathological stage of gastric carcinoma. These results suggest that OPRT levels were related to the histopathological characteristics of gastric carcinoma, and may be related to the response to fluoropyrimidine-based anticancer chemotherapy.
乳清酸磷酸核糖基转移酶（OPRT，EC 2.4.2.10）是与5-氟尿嘧啶第一步磷酸化过程相关的关键酶。我们最近开发了一种酶联免疫吸附测定（ELISA）系统来测量癌组织中的OPRT水平。我们使用该ELISA检测了75例胃癌组织中的OPRT水平，并评估了其与临床病理因素的关系。本研究共纳入75例手术切除的胃癌组织。肿瘤内OPRT水平通过新开发的酶联免疫吸附测定（ELISA）来确定。OPRT的酶活性也使用以放射性标记的5-氟尿嘧啶为底物的传统酶测定法来确定。通过ELISA测量的胃癌组织中的OPRT水平为5.4±3.6 ng/mg蛋白质，范围为0.2至15.7 ng/mg蛋白质。ELISA测量的OPRT水平与OPRT酶活性之间存在显著相关性（y = 0.545x - 0.017，r² = 0.617，p < 0.0001）。在分化型和浸润型胃癌患者中，OPRT水平显著更高，而OPRT水平与胃癌的病理分期无关。这些结果表明，OPRT水平与胃癌的组织病理学特征相关，并且可能与基于氟嘧啶的抗癌化疗反应有关。
