Saito Takeshi, Usui Noriko, Asai Osamu, Dobashi Nobuaki, Yano Shingo, Osawa Hiroshi, Takei Yutaka, Takahara Shinobu, Ogasawara Yoji, Otsubo Hiroko, Yamaguchi Yuko, Minami Jiro, Hoshi Yasutaka, Kataoka Motoyuki, Aiba Keisuke
Division of Oncology and Hematology, Department of Internal Medicine, Jikei University School of Medicine, 19-18 Nishishinbashi 3-chome, Minato-ku, Tokyo 105-8461, Japan.
J Infect Chemother. 2007 Dec;13(6):426-8. doi: 10.1007/s10156-007-0553-4. Epub 2007 Dec 25.
To investigate the role of matrix metalloproteinases (MMPs) in the mobilization of peripheral blood stem cells stimulated by granulocyte colony-stimulating factor (G-CSF), we analyzed MMP serum levels in 11 healthy donors and 9 patients who had hematological malignancies or germ cell tumors. A dose of 5-10 microg/kg per day of G-CSF (lenograstim) was administered for 4-8 days to each subject. The serum levels of MMP-2, and MMP-9; interleukin-3, -6, -8, and -10; stem cell factor; interferon-gamma; and tumor necrosis factor-alpha were measured both before and during G-CSF administration. MMP-9 was found to be increased in both the cancer patients and the healthy donor group. In contrast, the levels of each of the other factors tested were unchanged. No significant positive correlation was observed between the MMP-9 levels and the number of CD34+ cells. Hence, we found no significant role for MMPs during the mobilization of peripheral blood stem cells stimulated by G-CSF.
为研究基质金属蛋白酶（MMPs）在粒细胞集落刺激因子（G-CSF）刺激外周血干细胞动员中的作用，我们分析了11名健康供者以及9例血液系统恶性肿瘤或生殖细胞肿瘤患者的血清MMP水平。每位受试者每天给予5-10μg/kg剂量的G-CSF（来格司亭），持续4-8天。在给予G-CSF之前及期间，检测血清MMP-2、MMP-9、白细胞介素-3、-6、-8和-10、干细胞因子、干扰素-γ以及肿瘤坏死因子-α水平。结果发现，癌症患者组和健康供者组的MMP-9水平均升高。相比之下，所检测的其他各因子水平均未改变。MMP-9水平与CD34+细胞数量之间未观察到显著正相关。因此，我们发现MMPs在G-CSF刺激外周血干细胞动员过程中无显著作用。
