Kilemade Michael, Lemon Jennifer, Boreham Douglas
Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, Canada.
Environ Mol Mutagen. 2008 Apr;49(3):165-72. doi: 10.1002/em.20354.
The aim of this study was to investigate the influence of "priming" doses of ionizing irradiation on salmon cell survival in vitro prior to being challenged with subsequent higher doses. A radiation-induced adaptive response (AR) was examined in the Chinook salmon embryo cell line (CHSE-214). Cells were initially irradiated with a range of priming (conditioning) doses of (60)Co gamma (gamma) rays (0.25-0.75 Gy), followed by a challenge dose of 7.50 Gy at intervals of 24, 48, and 72 hr. The AR was assessed using a colony-forming assay. Cell survival was determined by counting the number of colonies (viable clones) after 40 days of culture. This study revealed that cells that received a priming dose of 0.50 Gy before delivering the higher challenge dose became more radiation resistant with an increase in cell survival of 29% over cells receiving the challenge dose alone. The cells showed maximum resistance to ionizing radiation when the priming dose was given 72 hr prior to the higher challenge dose. This study is one of the first to demonstrate an AR using an in vitro piscine system, and is generally consistent with other studies of both in vitro and in vivo systems across the taxa.
