Zheng Qing-yi, Hu An-gen, Zhang Min-feng, Chen Jin-he, Cai Shao-fu, Zheng Jian-sheng, Zhang Yang-gen
Department of Burns and Plastic Surgery, the 175th Hospital of PLA, Zhangzhou, P. R. China.
Zhonghua Shao Shang Za Zhi. 2007 Aug;23(4):249-52.
To explore the protective effect of high density lipoprotein on the lung function of rats with severe burns.
One hundred and thirty-five Wistar rats were employed in the study and were randomly divided into control (n = 15, without injury), burn (n = 60, with 30% TBSA full-thickness burn on the back) and experimental [(n = 60, with the injection of HDL (80 mg/kg) via the caudal vein immediately after burns)] groups. The rats in the latter two groups were resuscitated with intraperitoneal isotonic saline (50 ml/kg) 30 minutes after burns. The serum content of ICAM-1 and TNF-alpha as well as the blood content of PCO2 and PO2 of the rats in burn and experimental groups were determined at 12, 24, 48 and 72 post-burn hours (PBH) and in control group. The pathological changes in the lung tissue of the rats in all groups were observed under light microscope and electronic microscope at 48 PBH.
PCO2 and the contents of ICAM-1 and TNF-alpha in burn group were significantly higher, but the PO2 was lower than those in control group at each time-point (P < 0.05 or P < 0.01). There were no obvious differences in the above indices between the experimental and control groups (P > 0.05), but the ICAM-1 and TNF-alpha levels in experimental group were markedly decreased than those in burn group at each time-point (P < 0.05 or P < 0.01). The ICAM-1 and TNF-alpha contents in burn group at 48 PBH were (3.42 +/- 0.25) microg/L and (4. 04 +/- 0.28) ng/L, respectively, which were markedly higher than those in experimental group [(2.24 +/- 0.14) microg/L, (3.35 +/- 0.22) ng/L, P < 0.05 or P < 0.01]. Dilation of capillaries, congestion and inflammatory infiltration in the pulmonary capillaries, and loosening of conjunction between pulmonary capillary vascular endothelial cells and endothelial swelling were observed in burn group at 48 PBH. Compared with the burn group, the injury was markedly alleviated in the experiment group, and the pulmonary capillary endothelial cells showed tighter junction.
HDL exhibits a protective effect on the lung function of rats with severe burns via reducing the expression of ICAM-1 and TNF-alpha.
探讨高密度脂蛋白对重度烧伤大鼠肺功能的保护作用。
选取135只Wistar大鼠用于本研究，随机分为对照组（n = 15，未受伤）、烧伤组（n = 60，背部30%体表面积全层烧伤）和实验组（n = 60，烧伤后立即经尾静脉注射高密度脂蛋白（80 mg/kg））。后两组大鼠在烧伤后30分钟用腹腔等渗盐水（50 ml/kg）进行复苏。在烧伤后12、24、48和72小时（PBH）以及对照组测定烧伤组和实验组大鼠血清中ICAM - 1和TNF -α的含量以及血液中PCO2和PO2的含量。在烧伤后48小时用光学显微镜和电子显微镜观察所有组大鼠肺组织的病理变化。
烧伤组各时间点的PCO2以及ICAM - 1和TNF -α的含量均显著高于对照组，但PO2低于对照组（P < 0.05或P < 0.01）。实验组与对照组上述指标无明显差异（P > 0.05），但实验组各时间点的ICAM - 1和TNF -α水平均明显低于烧伤组（P < 0.05或P < 0.01）。烧伤组在烧伤后48小时的ICAM - 1和TNF -α含量分别为（3.42±0.25）μg/L和（4.04±0.28）ng/L，明显高于实验组[（2.24±0.14）μg/L，（3.35±0.22）ng/L，P < 0.05或P < 0.01]。烧伤组在烧伤后48小时可见肺毛细血管扩张、充血和炎症浸润，肺毛细血管血管内皮细胞连接疏松和内皮肿胀。与烧伤组相比，实验组损伤明显减轻，肺毛细血管内皮细胞连接紧密。
高密度脂蛋白通过降低ICAM - 1和TNF -α的表达对重度烧伤大鼠的肺功能具有保护作用。
