[The study in lasting effectivity on conditionally replicating adenovirus shRNA mediating survivin gene silencing in colon carcinoma cell line HT-29].

OBJECTIVE

To investigate the lasting effect of conditionally replicating adenovirus shRNA on survivin gene silencing in colon carcinoma cell lineHT-29.

METHODS

We transfected the colon cancer HT-29 cell with Ad-delE1b55KD-shRNA/Survivin-EGFP (control shRNA vector was the replication defective adenovirus called as Ad-shRNA/Survivin-EGFP). The expressions of EGFP, survivin mRNA and protein in HT-29 were detected at 1st day, 7th day, 14th day and 28th day after cell transfection.

RESULTS

The expression of EGFP in HT-29 was high at 7th day after cell transfection but survivin mRNA and protein expressions inhibited in each experiment group, among which Ad-delE1b55KD-shRNA/Survivin-EGFP group showed the most high EGFP expression; at 14th day, the expression of EGFP went down but the survivin mRNA and protein expressions upregulated obviously in replication defective adenovirus group and liposome vector group, however EGPF expression in HT-29 cell was still high and the survivin mRNA and protein expressions were still inhibited in Ad-delE1b55KD-shRNA/Survivin-EGFP group; at 28th day after cell HT-29 transfected, the expression of EGPF and the expression inhibition of survivin mRNA and protein disappeared in Ad-shRNA/Survivin-EGFP and liposome groups, but it was not in Ad-delE1b55KD-shRNA/Survivin-EGFP group (P < 0.05).

CONCLUSION

Conditionally replicating adenovirus shRNA can mediate the survivin gene silencing with long-lasting effects on colon carcinoma cell lines HT-29.