[Construction of the recombinant adenovirus vector bearing dual-survivin shRNA].

OBJECTIVE

To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).

METHODS

Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.

RESULTS

The recombinant adenovirus vector was constructed and the target sequence was obtained.

CONCLUSION

The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.