[Genetic polymorphism of interleukin-2 gene in Chinese Han and Thai populations].

OBJECTIVE

To investigate the polymorphisms of interleukin-2 (IL-2) gene at position -330 in the promoter region and +114 in the first exon, and to study the polymorphism distribution difference between the Chinese Han and Thai ethnic groups.

METHODS

Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, authors detected the single nucleotide polymorphisms at position -330T/G and +114G/T in IL-2 genes of 130 healthy Chinese Han and 105 healthy Thai individuals.

RESULTS

The frequency of TT genotype in IL-2 gene at position -330 was 36.9% in Chinese Han or 40.9% in Thai population. The frequency of TG genotype was 53.9% in Chinese Han or 39.1% in Thai population. The frequency of GG genotype was 9.2% in Chinese Han or 20.0% in Thai population. The frequencies of +114 GG, GT and TT genotypes were 20.8%, 55.8% and 23.3% respectively in Chinese Han, but 50.5%, 39.0% and 10.5% respectively in Thai population. The genotype frequencies of -330 and +114 were significantly different between Chinese Han and Thai population (P < 0.05). The genotype frequencies of -330 in Chinese Han were significantly different from those in Thai, Japanese and Spain ethnic groups (P > 0.05). The polymorphisms of + 114 in Chinese Han were significantly different from those in Thai and Spain (P < 0.05), but were similar with that in Japanese (P > 0.05).

CONCLUSION

IL-2 gene polymorphism in some different populations is significantly different. These differences may be one of the genetic factors contributing to the different pathogenesis, progress and prognosis in some diseases happening among ethnics or regions.