Waanders M M, Heidt S, Koekkoek K M, Zoet Y M, Doxiadis I I N, Amir A, Heemskerk M H M, Mulder A, Brand A, Roelen D L, Claas F H J
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Tissue Antigens. 2008 Jan;71(1):1-15. doi: 10.1111/j.1399-0039.2007.00979.x.
Monitoring of T cells involved in the alloimmune response after transplantation requires the availability of reliable in vitro assays for the detection of T cells with both direct and indirect allospecificity. While generally accepted assays exist to measure helper and cytotoxic T cells involved in direct allorecognition, consensus about an assay for monitoring indirect T-cell allorecognition in clinical transplantation is lacking. Many studies claim a relationship between the reactivity of T cells with indirect allospecificity and graft rejection, but different protocols are used and essential controls are often lacking. In this review, the disadvantages and pitfalls of the current approaches are discussed, in some cases supported by the results of our own in vitro experiments. We conclude that an international workshop is necessary to establish and validate a uniform, robust and reliable assay for the monitoring of transplant recipients and to study the actual role of indirect allorecognition in acute and chronic rejection.
移植后对参与同种免疫反应的T细胞进行监测，需要有可靠的体外检测方法，以检测具有直接和间接同种特异性的T细胞。虽然存在普遍认可的检测方法来测量参与直接同种异体识别的辅助性T细胞和细胞毒性T细胞，但在临床移植中，对于监测间接T细胞同种异体识别的检测方法尚未达成共识。许多研究声称，具有间接同种特异性的T细胞反应性与移植排斥之间存在关联，但使用的方案各不相同，而且往往缺乏关键对照。在本综述中，我们讨论了当前方法的缺点和陷阱，在某些情况下，我们自己的体外实验结果也支持了这些观点。我们得出结论，有必要举办一次国际研讨会，以建立和验证一种统一、稳健且可靠的检测方法，用于监测移植受者，并研究间接同种异体识别在急性和慢性排斥反应中的实际作用。
