Setiyaningsih Surachmi, Desport Moira, Stewart Meredith E, Hartaningsih Nining, Wilcox Graham E
School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Perth, Western Australia 6150, Australia.
Virus Res. 2008 Mar;132(1-2):220-5. doi: 10.1016/j.virusres.2007.11.004. Epub 2007 Dec 21.
Jembrana disease virus (JDV) is a lentivirus which replicates to very high titres in vivo and its Tat-1 protein has been shown to be a potent transactivator in vitro. Analysis of tat mRNA transcripts produced early in infection studies identified four predominant species which were generated by multiple splicing events. The use of a splice donor downstream of tat-1 was common indicating that a Tat-1 protein of 97 amino acids is expressed during the acute phase of Jembrana disease. The presence of an in-frame stop codon between tat-1 and tat-2 was identified in transcripts from three different strains of JDV confirming that expression of a single exon Tat-1 correlates with high viral titres in vivo. Sequence conservation in the regions of tat-1 that are critical for RNA binding and transcription activation in three different virus strains was high and the tat-2 sequences were completely conserved.
杰姆纳纳病病毒（JDV）是一种慢病毒，它在体内可复制至非常高的滴度，并且其Tat-1蛋白在体外已被证明是一种有效的反式激活因子。在感染研究早期产生的tat mRNA转录本分析中，鉴定出了四种主要的转录本，它们是由多个剪接事件产生的。在tat-1下游使用剪接供体很常见，这表明在杰姆纳纳病急性期会表达一种97个氨基酸的Tat-1蛋白。在来自三种不同JDV毒株的转录本中，鉴定出tat-1和tat-2之间存在一个框内终止密码子，这证实了单个外显子Tat-1的表达与体内高病毒滴度相关。在三种不同病毒毒株中，tat-1区域对RNA结合和转录激活至关重要的区域序列保守性很高，并且tat-2序列完全保守。
