Nazarko Volodymyr Y, Thevelein Johan M, Sibirny Andriy A
Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
Cell Biol Int. 2008 May;32(5):502-4. doi: 10.1016/j.cellbi.2007.11.001. Epub 2007 Nov 12.
In yeast cell, glucose induces various changes of cellular metabolism on genetic and metabolic levels. One of such changes is autophagic degradation of dispensable peroxisomes (pexophagy) which occurs in vacuoles. We have found that in Saccharomyces cerevisiae, defect of G-protein-coupled receptor Gpr1 and G-protein Gpa2, both the components of cAMP-signaling pathway, strongly suppressed glucose-induced degradation of matrix peroxisomal protein thiolase. We conclude that proteins Gpr1 and Gpa2 are involved in glucose sensing and signal transduction during pexophagy process in yeast.
在酵母细胞中，葡萄糖会在基因和代谢水平上引发细胞代谢的各种变化。其中一种变化是在液泡中发生的可降解过氧化物酶体的自噬降解（过氧化物酶体自噬）。我们发现，在酿酒酵母中，cAMP信号通路的两个组成部分——G蛋白偶联受体Gpr1和G蛋白Gpa2的缺陷，强烈抑制了葡萄糖诱导的基质过氧化物酶体蛋白硫解酶的降解。我们得出结论，蛋白Gpr1和Gpa2在酵母过氧化物酶体自噬过程中的葡萄糖感知和信号转导中发挥作用。
