Ding Caifeng, Zhao Fei, Zhang Meiling, Zhang Shusheng
Key Laboratory of Eco-chemical Engineering, Ministry of Education; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Bioelectrochemistry. 2008 Feb;72(1):28-33. doi: 10.1016/j.bioelechem.2007.11.001. Epub 2007 Nov 12.
A label-free biosensor for the detection of oligonucleotides related to hepatitis B virus sequence via the interactions of DNA with redox-active complex, 2,9-dimethyl-1,10-phenantroline cobalt [Co(dmp)(H2O)(NO3)2] is described. The study was carried out by the hybridization of 21-mer probe DNA modified on glassy carbon electrode (GCE) with target DNA, and [Co(dmp)(H2O)(NO3)2] whose sizes are comparable to those of the small groove of native double-helix DNA was used as an electrochemical indicator. Electrochemical detection was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) over the potential range where the [Co(dmp)(H2O)(NO3)2] was active. Under the optimum conditions, the electrical signal had a linear relationship with the concentration of target DNA ranging from 3.96 x 10(-7) to 1.32 x 10(-6) M, and the detection limit was 1.94 x 10(-8) M (S/N=3). The biosensor has good selectivity by detecting the three-base mismatch sequence ssDNA.
描述了一种无标记生物传感器，用于通过DNA与氧化还原活性配合物2,9-二甲基-1,10-菲咯啉钴[Co(dmp)(H2O)(NO3)2]的相互作用来检测与乙型肝炎病毒序列相关的寡核苷酸。该研究通过将修饰在玻碳电极(GCE)上的21聚体探针DNA与靶DNA杂交进行，并且使用尺寸与天然双螺旋DNA小沟尺寸相当的[Co(dmp)(H2O)(NO3)2]作为电化学指示剂。在[Co(dmp)(H2O)(NO3)2]具有活性的电位范围内，通过循环伏安法(CV)和差分脉冲伏安法(DPV)进行电化学检测。在最佳条件下，电信号与靶DNA浓度在3.96×10(-7)至1.32×10(-6) M范围内呈线性关系，检测限为1.94×10(-8) M (S/N=3)。该生物传感器通过检测三碱基错配序列单链DNA具有良好的选择性。
