Yarke Cory A, Dalheimer Stacy L, Zhang Na, Catron Drew M, Jenkins Marc K, Mueller Daniel L
Department of Medicine, Center for Immunology, University of Minnesota Medical School, Minneapolis 55455, USA.
J Immunol. 2008 Jan 1;180(1):156-62. doi: 10.4049/jimmunol.180.1.156.
To investigate the role of TCR signaling in the exit of CD4+ T cells from cell cycle, we took advantage of a low frequency TEa T cell adoptive transfer technique as well as the Y-Ae mAb to interrupt Ag/MHC recognition before the completion of clonal expansion. Termination of TCR signaling after 36 h of Ag exposure caused an immediate reduction in cell size and deceleration of G1->SG2M phase cell cycle progression. As a consequence, clonal expansion in the absence of durable TCR signaling decreased by two-thirds. Thus, CD4+ T cells scan for the presence Ag throughout their clonal expansion response, and continuously adjust their rate of cell growth and G1->S phase transition to match their intensity of TCR signaling.
为了研究TCR信号在CD4⁺ T细胞退出细胞周期中的作用，我们利用低频TEa T细胞过继转移技术以及Y-Ae单克隆抗体在克隆扩增完成前中断抗原/主要组织相容性复合体（Ag/MHC）的识别。抗原暴露36小时后TCR信号的终止导致细胞大小立即减小，并且G1期到S/G2M期的细胞周期进程减速。结果，在缺乏持久TCR信号的情况下，克隆扩增减少了三分之二。因此，CD4⁺ T细胞在整个克隆扩增反应过程中搜寻抗原的存在，并不断调整其细胞生长速率和G1期到S期的转变，以匹配其TCR信号强度。
