来自苜蓿中华根瘤菌CECT4114的活性位点突变海因消旋酶的结晶及初步晶体学研究。
Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.
作者信息
Martínez-Rodríguez Sergio, González-Ramírez Luis Antonio, Clemente-Jiménez Josefa María, Rodríguez-Vico Felipe, Las Heras-Vázquez Francisco Javier, Gavira Jose Antonio, García-Ruiz Juan Ma
机构信息
Department of Cellular and Molecular Interactions, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):50-3. doi: 10.1107/S1744309107066122. Epub 2007 Dec 20.
A recombinant active-site mutant of hydantoin racemase (C76A) from Sinorhizobium meliloti CECT 4114 (SmeHyuA) has been crystallized in the presence and absence of the substrate D,L-5-isopropyl hydantoin. Crystals of the SmeHyuA mutant suitable for data collection and structure determination were grown using the counter-diffusion method. X-ray data were collected to resolutions of 2.17 and 1.85 A for the free and bound enzymes, respectively. Both crystals belong to space group R3 and contain two molecules of SmeHyuA per asymmetric unit. The crystals of the free and complexed SmeHyuA have unit-cell parameters a = b = 85.43, c = 152.37 A and a = b = 85.69, c = 154.38 A, crystal volumes per protein weight (V(M)) of 1.94 and 1.98 A3 Da(-1) and solvent contents of 36.7 and 37.9%, respectively.
来自苜蓿中华根瘤菌CECT 4114的乙内酰脲消旋酶（C76A）的重组活性位点突变体（SmeHyuA）已在存在和不存在底物D，L-5-异丙基乙内酰脲的情况下结晶。使用反扩散法培养出适合数据收集和结构测定的SmeHyuA突变体晶体。分别为游离酶和结合酶收集了分辨率为2.17 Å和1.85 Å的X射线数据。两种晶体均属于空间群R3，每个不对称单元包含两个SmeHyuA分子。游离和复合SmeHyuA的晶体的晶胞参数分别为a = b = 85.43，c = 152.37 Å和a = b = 85.69，c = 154.38 Å，每蛋白质重量的晶体体积（V(M)）为1.94和1.98 Å3 Da(-1)，溶剂含量分别为36.7%和37.9%。
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