Sun Xiaodong, Xu Xun, Wang Fenghua, Zhang Xi, Ho Patrick C P, Liu Haiyang, Qian Jin, Yu Zhang, Lu Hongfen, Xu Weiqi
Eye Research Institute of Shanghai Jiaotong University, Shanghai, China.
Ophthalmologica. 2008;222(1):58-61. doi: 10.1159/000109281. Epub 2007 Dec 19.
To investigate the expression of nerve growth factor (NGF) and its receptor TrkA on the retina at different time points after retinal detachment (RD) and the protection effect of NGF in experimental RD.
Sprague-Dawley rats were used as an RD animal model by injection of 0.1% sodium hyaluronate under the neurosensory retina. The expression of endogenous NGF and its receptor TrkA in the rat retina was detected using immunohistochemistry. The NGF (5 mug/eye) or phosphate-buffered saline were injected separately into vitreous every 4 days after the RD. The rat eyes were then observed at various time points. Morphologic changes were investigated by light microscopy. Retinal gliosis was detected by glial fibrillary acidic protein labeling.
The expression of endogenous NGF and TrkA was upregulated during RD procedure. Most of the NGF-treated retina had a well-organized structure. In NGF-treated RD eyes, the cells were still significantly more numerous than in phosphate-buffered-saline-treated retina. Glial fibrillary acidic protein labeling increased quickly after RD; the NGF-treated retina had less reactive gliosis than the control groups.
Intravitreal injection of exogenous NGF can protect retinal cells from degeneration in experimental RD. It may exert its protective action by preventing the apoptosis of retinal cells after RD.
研究视网膜脱离（RD）后不同时间点神经生长因子（NGF）及其受体TrkA在视网膜上的表达情况，以及NGF在实验性RD中的保护作用。
通过在神经感觉视网膜下注射0.1%透明质酸钠，将Sprague-Dawley大鼠作为RD动物模型。采用免疫组织化学法检测大鼠视网膜内源性NGF及其受体TrkA的表达。RD后每4天向玻璃体腔分别注射NGF（5μg/眼）或磷酸盐缓冲液。然后在不同时间点观察大鼠眼睛。通过光学显微镜观察形态学变化。通过胶质纤维酸性蛋白标记检测视网膜胶质增生。
在RD过程中内源性NGF和TrkA的表达上调。大多数接受NGF治疗的视网膜结构组织良好。在接受NGF治疗的RD眼中，细胞数量仍明显多于接受磷酸盐缓冲液治疗的视网膜。RD后胶质纤维酸性蛋白标记迅速增加；接受NGF治疗的视网膜比对照组的反应性胶质增生更少。
玻璃体内注射外源性NGF可保护实验性RD中的视网膜细胞免于退变。它可能通过防止RD后视网膜细胞凋亡发挥其保护作用。
