Cao Shao-Feng, Li Ding, Yuan Qing, Guan Xin, Xu Chen
Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Asian J Androl. 2008 Mar;10(2):277-85. doi: 10.1111/j.1745-7262.2008.00324.x. Epub 2007 Dec 20.
To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development.
Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70.
c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate. c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter.
The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis.
对c-mos基因产物进行免疫定位，并研究其在小鼠出生后发育过程中睾丸内的时空表达情况。
采用半定量逆转录-聚合酶链反应（RT-PCR）和原位杂交技术检测c-mos mRNA，并用间接免疫荧光法对出生后第14、21、25、28、30、35、49和70天的小鼠睾丸中的c-Mos蛋白进行定位。
c-mos mRNA在出生后第14 - 21天保持低水平，从第25天开始急剧增加，并在第30天达到峰值。其水平在第35天略有下降，此后直至第70天几乎保持稳定。c-mos mRNA定位于精母细胞和圆形精子细胞的细胞核和细胞质中。核染色比细胞质染色强得多。使用多克隆抗c-Mos抗体，蛋白质印迹法在睾丸裂解物中检测到一条43 kDa的单带。c-Mos蛋白仅定位于伸长的精子细胞，在出生后第30天首次检测到。c-Mos阳性精子细胞的数量逐渐增加直至第49天，此后稳定下来。
c-mos基因在小鼠出生后发育过程中，在睾丸的mRNA和蛋白质水平上均呈现出时空表达模式。这表明c-mos可能在精子发生过程中发挥重要作用。
