Chen Kuaikuai, Hu Hongbo, Wang Wei, Zhang Xuehong, Xu Yuquan
Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P.R. China.
Biodegradation. 2008 Sep;19(5):659-67. doi: 10.1007/s10532-007-9171-1. Epub 2007 Dec 21.
The biotransformation of phenazine-1-carboxylic acid (PCA) by PCA-degrading strain Sphingomonas sp. DP58 yielded small quantities of metabolites and was demonstrated for the first time. The metabolites were isolated by using preparative high-performance liquid chromatography (HPLC). In addition, these were subsequently characterized by gas chromatography (GC)-mass spectrum (MS) after N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatization and (1)H-nuclear magnetic resonance (NMR). They were identified as 4-hydroxy-1-(2-carboxyphenyl) azacyclobut-2-ene-2-carbonitrile (HPAEC) and 4-hydroxy-1-(2-carboxyphenyl)-2-azetidinecarbonitrile (HPAC). The two metabolites had transformational relationship between each other.
吩嗪-1-羧酸（PCA）降解菌株鞘氨醇单胞菌属DP58对PCA的生物转化产生了少量代谢产物，这是首次得到证实。代谢产物通过制备型高效液相色谱（HPLC）进行分离。此外，这些代谢产物在经过N,O-双（三甲基硅基）三氟乙酰胺（BSTFA）衍生化后，通过气相色谱（GC）-质谱（MS）以及氢核磁共振（¹H-NMR）进行了后续表征。它们被鉴定为4-羟基-1-（2-羧基苯基）氮杂环丁-2-烯-2-腈（HPAEC）和4-羟基-1-（2-羧基苯基）-2-氮杂环丁烷腈（HPAC）。这两种代谢产物相互之间存在转化关系。
