Chtcheglova Lilia A, Haeberli André, Dietler Giovanni
Laboratoire de Physique de la Matière Vivante, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Biopolymers. 2008 Apr;89(4):292-301. doi: 10.1002/bip.20910.
Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s.
纤维蛋白聚合在许多生理和病理过程中至关重要，如血液凝固、伤口愈合和血栓形成。在本研究中，我们使用原子力显微镜通过力谱研究了纤维蛋白原 - 纤维蛋白聚合初始步骤中涉及的力。我们的数据证实了纤维蛋白与纤维蛋白（原）之间存在强烈的特异性相互作用，解离力范围为290至375皮牛，并且在0.8至23纳牛/秒的加载速率之间呈对数依赖性。
