对大肠杆菌K12硝酸还原酶中钼的电子顺磁共振研究。
Electron-paramagnetic-resonance studies on the molybdenum of nitrate reductase from Escherichia coli K12.
作者信息
Bray R C, Vincent S P, Lowe D J, Clegg R A, Garland P B
出版信息
Biochem J. 1976 Apr 1;155(1):201-3. doi: 10.1042/bj1550201.
Abstract
Studies on the respiratory nitrate reductase (EC 1.7.99.4) from Escherichia coli K12 by electron-paramagnetic-resonance spectroscopy indicate that its molybdenum centre is comparable with that in other molybdenum-containing enzymes. Two Mo(V) signals may be observed; one shows interaction of Mo(V) with a proton exchangeable with the solvent and has: A (1H) 0.9-1.2mT; g1 = 1.999; g2=1.985; g3 = 1.964; gav. = 1.983. Molybdenum of both signal-giving species may be reduced with dithionite and reoxidized with nitrate.
摘要
通过电子顺磁共振光谱对大肠杆菌K12的呼吸硝酸盐还原酶（EC 1.7.99.4）进行的研究表明，其钼中心与其他含钼酶中的钼中心相当。可以观察到两个Mo(V)信号；其中一个显示Mo(V)与可与溶剂进行质子交换的质子之间的相互作用，其具有：A(1H) 0.9 - 1.2mT；g1 = 1.999；g2 = 1.985；g3 = 1.964；gav. = 1.983。这两种产生信号的物种的钼都可以用连二亚硫酸盐还原，并用硝酸盐再氧化。
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本文引用的文献
1
Nitrate reductase from Pseudomonas aeruginosa.来自铜绿假单胞菌的硝酸还原酶。
Biochim Biophys Acta. 1961 May 13;49:335-49. doi: 10.1016/0006-3002(61)90133-0.
2
Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase.钼与硝酸还原酶。II. 作为硝酸还原酶组成成分的钼。
J Biol Chem. 1954 Mar;207(1):353-60.
3
Hepatic sulfite oxidase. A functional role for molybdenum.肝亚硫酸盐氧化酶。钼的功能作用。
J Biol Chem. 1971 Jan 25;246(2):374-82.
4
Proposed molecular mechanism for the action of molybedenum in enzymes: coupled proton and electron transfer.钼在酶中作用的拟议分子机制：质子与电子的耦合转移。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):988-92. doi: 10.1073/pnas.70.4.988.
5
The mechanism of action of xanthine oxidase.黄嘌呤氧化酶的作用机制。
J Biol Chem. 1974 Jul 25;249(14):4363-82.
6
Spin-spin interaction between molybdenum and one of the iron-sulphur systems of xanthine oxidase and its relevance to the enzymic mechanism.钼与黄嘌呤氧化酶的铁硫系统之一之间的自旋-自旋相互作用及其与酶促机制的相关性。
Biochem J. 1972 Nov;130(1):239-49. doi: 10.1042/bj1300239.
7
The bacterial nitrate reductases: EPR studies on nitrate reductase A from Micrococcus denitrificans.细菌硝酸还原酶：对反硝化微球菌硝酸还原酶A的电子顺磁共振研究。
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8
Electron spin resonance of xanthine oxidase substituted with molybdenum-95.用钼-95取代的黄嘌呤氧化酶的电子自旋共振
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