Wiedłocha A, Paprocka M, Radzikowski C
Department of Tumor Immunology, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1991;39(3):235-41.
The purging of mouse bone marrow from L1210V leukemic cells was attained ex vivo by using monoclonal IgM antibody against leukemic cells and magnetic particles charged with goat anti-mouse IgM antibodies (immunobeads), followed by magnetic separation. The monoclonal antibody MoAb-16 recognizes oncofetal antigen on target cells and binds to 100% L1210V cells. The number of the remaining clonogenic leukemic cells was examined by LCFU and bioassay tests. The clonogenic capacity of the bone marrow progenitor cells was measured by NCFU test. After first treatment cycle the number of LCFU was reduced in a dose-dependent manner. After second cycle of purification a complete elimination of L1210V cells from marrow was achieved at the concentration 10 mg/ml of magnetic particles. Although at lower concentrations of immunobeads no leukemic spleen colonies were found, some residual L1210V cells were still present in the bone marrow and were able to grow and kill the recipient mice. The loss of bone marrow progenitor cells during magnetic removal procedure was about 10%.
通过使用抗白血病细胞的单克隆IgM抗体和负载山羊抗小鼠IgM抗体的磁性颗粒（免疫磁珠），随后进行磁分离，在体外实现了从L1210V白血病细胞中清除小鼠骨髓。单克隆抗体MoAb - 16识别靶细胞上的癌胚抗原，并与100%的L1210V细胞结合。通过低剂量集落形成单位（LCFU）和生物测定试验检测剩余的克隆形成白血病细胞数量。通过中性集落形成单位（NCFU）试验测量骨髓祖细胞的克隆形成能力。在第一个治疗周期后，LCFU的数量呈剂量依赖性减少。在第二个纯化周期后，在磁性颗粒浓度为10 mg/ml时，实现了从骨髓中完全清除L1210V细胞。尽管在较低浓度的免疫磁珠下未发现白血病脾集落，但骨髓中仍存在一些残留的L1210V细胞，这些细胞能够生长并杀死受体小鼠。磁去除过程中骨髓祖细胞的损失约为10%。
