Villar M J, Ceccatelli S, Hökfelt T
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Neuroreport. 1991 Nov;2(11):703-6. doi: 10.1097/00001756-199111000-00018.
Using immunofluorescence histochemistry, the paraventricular and supraoptic hypothalamic nuclei of normal control and hypophysectomized rats were studied in double labelling experiments with antibodies against the protein c-fos (Fos) and against vasopressin or oxytocin in order to characterize the activated neurons chemically. Normal controls showed no expression of Fos, whereas in hypophysectomized animals an intense induction of Fos-like immunoreactivity (-LI) was observed 12 h and 24 h post hypophysectomy but not beyond this survival time. Both vasopressinergic and oxytocinergic magnocellular neurons were labelled with Fos-LI. Thus Fos-LI can be induced in magnocellular hypothalamic neurons by injury, suggesting that this protein may be involved in adaptive mechanisms following axotomy.
利用免疫荧光组织化学技术，在正常对照大鼠和垂体切除大鼠中，通过针对原癌基因c-fos（Fos）蛋白、抗加压素或抗催产素的抗体进行双标记实验，对下丘脑室旁核和视上核进行了研究，以便从化学角度表征被激活的神经元。正常对照大鼠未显示Fos表达，而在垂体切除的动物中，垂体切除术后12小时和24小时观察到Fos样免疫反应性（-LI）的强烈诱导，但在此存活时间之后未观察到。加压素能和催产素能大细胞神经元均被Fos-LI标记。因此，损伤可在下丘脑大细胞神经元中诱导Fos-LI，这表明该蛋白可能参与轴突切断后的适应性机制。
