Moshkov D A, Saveljeva L N, Yanjushina G V, Funtikov V A
Institute of Biological Physics, USSR Academy of Sciences, Pushchino.
Acta Histochem Suppl. 1991;41:241-7.
The ultrastructure and protein content of goldfish Mauthner cells (M-cells) at different functional states induced by natural vestibular stimulations were studied. 2-h stimulation, usually causing a fatiguing of the fishes, was found to be accompanied by ultrastructural changes within M-cells and a decreased content of cytoskeletal proteins. After training by short stimulations resulting in a long-term adaptation of the fishes, the ultrastructure and protein content of M-cells could not be distinguished qualitatively and quantitatively from those of non-adapted fishes. When the adapted fishes were stimulated for 2 h the content of a 70-kDa protein was found to be increased. In addition, the content of a 42-kDa protein, obviously actin, was elevated in this case. Correspondingly, electron microscopic analysis demonstrated a significantly increased resistance of the cytoskeleton to fatiguing stimulation. The data obtained indicate that the neural cytoskeleton is a central target of fatiguing stimulation. We suppose that the 70-kDa protein is responsible for the adaptive properties of the cytoskeleton. This protein is assumed to be identical with one of the so-called heat-shock proteins of non-neural cells which have the same electrophoretic mobility and are also able to protect the cytoskeleton under stress conditions.
研究了自然前庭刺激诱导的不同功能状态下金鱼毛特纳细胞（M细胞）的超微结构和蛋白质含量。发现持续2小时的刺激通常会使鱼疲劳，同时伴随着M细胞内的超微结构变化以及细胞骨架蛋白含量的降低。经过短期刺激训练使鱼产生长期适应性后，M细胞的超微结构和蛋白质含量在定性和定量上与未适应的鱼没有区别。当对适应后的鱼进行2小时刺激时，发现一种70 kDa蛋白质的含量增加。此外，在这种情况下，一种明显为肌动蛋白的42 kDa蛋白质的含量也升高了。相应地，电子显微镜分析表明细胞骨架对疲劳刺激的抵抗力显著增强。所获得的数据表明神经细胞骨架是疲劳刺激的主要靶点。我们推测70 kDa蛋白质负责细胞骨架的适应性特性。这种蛋白质被认为与非神经细胞的一种所谓热休克蛋白相同，它们具有相同的电泳迁移率，并且在应激条件下也能够保护细胞骨架。
