Lee C Y
Endocrinology. 1976 Jul;99(1):42-8. doi: 10.1210/endo-99-1-42.
Specific binding of human chorionic gonadotropin (hCG), and the hCG-sensitive adenyl cyclase of granulosa cells from small (1-2 mm), medium (3-5 mm), and large (6-12 mm) porcine ovarian follicles have been studied. The number of hCG-binding sites per cell (n) increases during follicle maturation without a change in the binding affinity. The values for n were 300-400 for small, 1,000-1,600 for medium, and 8,200-10,000 for large-follicle cells. The dissociation constant is 2.4 X 10(-10)M for all cells. hCG-sensitive adenyl cyclase was demonstrated in porcine granulosa cells. The adenyl cyclase system of granulosa cells becomes increasingly responsive to hCG stimulation during follicle development. Maximal adenyl cyclase activation by hCG (1 mug/ml) was 240, 750, and 7,000 molecules of cyclic AMP formed/sec/cell, respectively, for small, medium, and large follicle cell. The concentration of hCG giving half-maximal stimulation (1.0 X 10(-9)M) was similar for both large and medium follicle cells. It is concluded that: 1) an increase in hCG receptor sites per cell occurs during maturation of the porcine ovarian follicle without change of binding affinity, and 2) the increase in the number of hCG receptors correlates well with hCG-sensitive adenyl cyclase activity during follicle development.
对人绒毛膜促性腺激素（hCG）以及来自小型（1 - 2毫米）、中型（3 - 5毫米）和大型（6 - 12毫米）猪卵巢卵泡颗粒细胞中对hCG敏感的腺苷酸环化酶的特异性结合进行了研究。每个细胞上hCG结合位点的数量（n）在卵泡成熟过程中增加，而结合亲和力不变。小型卵泡细胞的n值为300 - 400，中型为1000 - 1600，大型卵泡细胞为8200 - 10000。所有细胞的解离常数均为2.4×10⁻¹⁰M。在猪颗粒细胞中证实了对hCG敏感的腺苷酸环化酶。在卵泡发育过程中，颗粒细胞的腺苷酸环化酶系统对hCG刺激的反应性越来越高。对于小型、中型和大型卵泡细胞，hCG（1微克/毫升）刺激产生的最大腺苷酸环化酶活性分别为每秒每细胞形成240、750和7000个环磷酸腺苷分子。对于大型和中型卵泡细胞，产生半最大刺激的hCG浓度（1.0×10⁻⁹M）相似。得出以下结论：1）在猪卵巢卵泡成熟过程中，每个细胞上的hCG受体位点数量增加，而结合亲和力不变；2）在卵泡发育过程中，hCG受体数量的增加与对hCG敏感的腺苷酸环化酶活性密切相关。
