Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks.

A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH groups, NAD or NADP as coenzymes, and had an optimum at about pH 7-8. The enzyme's activity against 17 different monosaccharides and their derivatives was tested. The best substrate was beta-D-glucose, followed by D-glucose, D-glucosamine and D-xylose. Other compounds such as D-glucose-6-phosphate, D-fructose, D-mannose and 2-deoxyglucose were not oxidized. The enzyme preparation showed presence of enzymes which conduct gluconic acid metabolism.