Danisová A, Kolena J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Endocr Regul. 1991 Dec;25(4):207-10.
The involvement of cytoskeletal proteins in the gonadotropic regulation of granulosa cell progesterone (P4) and luteinization stimulator (LS) secretion was studied. The effect of cytochalasin B (CB) and colchicine (Col) on LS secretion was assessed in 4-day culture of porcine granulosa cells isolated from medium and large size follicles (MGC+LGC). The amount of LS released into the culture medium (CM) was determined by adding this LS-enriched CM to the cultures of granulosa cells isolated from small follicles (SGC) and by measuring P4 produced by them. Col (0.1 and 100 mumol l-1 and CB 2.0 mumol l-1) inhibited P4 synthesis by SGC. The secretion of LS was significantly reduced by Col at both concentrations used, but not by CB. These findings indicate specific involvement of microtubules in the secretion of luteinization stimulator by granulosa cells.
研究了细胞骨架蛋白在颗粒细胞孕酮（P4）促性腺调节及黄体化刺激因子（LS）分泌中的作用。在从中等大小和大卵泡分离的猪颗粒细胞（MGC + LGC）的4天培养中，评估了细胞松弛素B（CB）和秋水仙碱（Col）对LS分泌的影响。通过将富含LS的培养基添加到从小卵泡分离的颗粒细胞（SGC）培养物中，并测量它们产生的P4，来确定释放到培养基（CM）中的LS量。Col（0.1和100 μmol l-1）和CB（2.0 μmol l-1）抑制SGC的P4合成。两种浓度的Col均显著降低了LS的分泌，但CB没有。这些发现表明微管在颗粒细胞黄体化刺激因子的分泌中具有特定作用。
