三苯基锡对小鼠胸腺细胞的细胞毒性作用：一项使用荧光染料检测膜电位和细胞内钙离子的流式细胞术研究

Cytotoxic action of triphenyltin on mouse thymocytes: a flow-cytometric study using fluorescent dyes for membrane potential and intracellular Ca2+.

作者信息

Oyama Y, Chikahisa L, Tomiyoshi F, Hayashi H

机构信息

Department of Health Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.

出版信息

Jpn J Pharmacol. 1991 Nov;57(3):419-24. doi: 10.1254/jjp.57.419.

DOI:10.1254/jjp.57.419

PMID:1813667

Abstract

Effects of triphenyltin on mouse thymocytes were examined using fluorescent dyes to monitor membrane potential and intracellular Ca2+. Triphenyltin at 3 x 10(-7) M to 1 x 10(-6) M hyperpolarized thymocytes and depolarized them at 3 x 10(-6) M or more, associated with increasing intracellular Ca2+. Hyperpolarization was suppressed by quinine, but not by tetraethylammonium and 4-aminopyridine, suggesting the involvement of Ca(2+)-activated K+ current. Triphentyltin failed to hyperpolarize thymocytes in Ca(2+)-free solution. Results indicate that triphenyltin promotes Ca(2+)-influx to thymocytes. Such an action of triphenyltin may be related to the immunotoxicity of organotins.

摘要

使用荧光染料监测膜电位和细胞内钙离子，研究三苯基锡对小鼠胸腺细胞的影响。浓度为3×10⁻⁷ M至1×10⁻⁶ M的三苯基锡使胸腺细胞超极化，而浓度为3×10⁻⁶ M及以上时则使其去极化，同时细胞内钙离子增加。奎宁可抑制超极化，但四乙铵和4-氨基吡啶则不能，提示存在钙激活钾电流。在无钙溶液中，三苯基锡不能使胸腺细胞超极化。结果表明，三苯基锡促进钙离子流入胸腺细胞。三苯基锡的这种作用可能与有机锡的免疫毒性有关。