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[Cloning a species-specific DNA probe from Fusarium oxysporum fungus].

作者信息

Irisbaev B K, Kraev A S, Pozmogova G E, Abdukarimov A A, Skriabin K G

出版信息

Mol Biol (Mosk). 1991 Nov-Dec;25(6):1667-9.

PMID:1813809
Abstract

A method to obtain genus-specific DNA probes is suggested. It consists of specific amplification of the intergenic spacer between the 18S and 5.8S ribosomal RNA genes, using primers deduced from conservative ribosomal DNA sequences. The utility of the method is demonstrated on isolation of the 209 b.p. spacer fragment from the genomic DNA of a plant pathogenic fungus Fusarium oxysporum.

摘要

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