Gottlieb C, Svanborg K, Bygdeman M
Department of Obstetrics and Gynecology, Karolinska Hospital, Stockholm, Sweden.
Andrologia. 1991 Nov-Dec;23(6):421-5. doi: 10.1111/j.1439-0272.1991.tb02591.x.
The seminal adenosine triphosphate (ATP) content was determined by bioluminescence after treatment with trichloroacetic acid (TCA) in 81 semen samples 1.5 h after ejaculation obtained from men attending our fertility clinic, and selected to contain either 20% or less spermatozoa with good progressive motility (n = 22), or 60% or more spermatozoa with good progressive motility (n = 59) (Study I), and in 18 semen samples from fertile men 30 min and 3.5 h after ejaculation (Study II). The latter samples were divided into 2 equally large groups according to sperm motility. In Study I the mean sperm ATP concentration was significantly higher in the semen samples with bad motility (0.63 nmol per living spermatozoa x 10(-6)) than in semen samples with good motility (0.39 nmol per living spermatozoa x 10(-6); P less than 0.01). In Study II the ATP concentration per living spermatozoa was also lower in the group with the best motility in comparison with the spermatozoa with lower motility (P less than 0.01), both 30 min and 3.5 h after ejaculation. During the 3-5 h incubation the sperm ATP concentration decreased by 21% (P less than 0.01) in the former group of samples but remained unchanged in the latter group. The results indicate that, in semen samples with highly motile spermatozoa, the consumption of ATP is higher than in semen samples with impaired sperm motility. It is therefore essential that the time between ejaculation and ATP measurement is as short as possible to obtain comparable results. Repeated ATP measurements in combination with an analysis of the number of living spermatozoa, may provide further information on the fertilizing capacity of spermatozoa.
对从我院生殖门诊就诊男性获取的81份射精后1.5小时的精液样本，用三氯乙酸（TCA）处理后通过生物发光法测定精液中三磷酸腺苷（ATP）含量。这些样本被分为两组，一组含有20%或更少具有良好前向运动能力的精子（n = 22），另一组含有60%或更多具有良好前向运动能力的精子（n = 59）（研究I）；还对18份来自生育力正常男性射精后30分钟和3.5小时的精液样本进行了研究（研究II）。后一组样本根据精子活力分为两个同等规模的组。在研究I中，精子活力差的精液样本中平均精子ATP浓度（每存活精子0.63 nmol×10⁻⁶）显著高于精子活力好的精液样本（每存活精子0.39 nmol×10⁻⁶；P < 0.01）。在研究II中，射精后30分钟和3.5小时，活力最佳组的每存活精子ATP浓度也低于活力较低组的精子（P < 0.01）。在3 - 5小时的孵育过程中，前一组样本中的精子ATP浓度下降了21%（P < 0.01），而后一组保持不变。结果表明，在精子活力高的精液样本中，ATP的消耗高于精子活力受损的精液样本。因此，为获得可比结果，射精与ATP测量之间的时间应尽可能短。结合分析存活精子数量重复进行ATP测量，可能会提供关于精子受精能力的更多信息。
