McCarron R M, Wang L, Cowan E P, Spatz M
Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Brain Res. 1991 Dec 6;566(1-2):325-8. doi: 10.1016/0006-8993(91)91718-g.
Cerebral vascular endothelial cells (EC) isolated from human brain do not constitutively express class II MHC antigens. However, incubation in the presence of human interferon-gamma (IFN gamma) resulted in the expression of both HLA-DR and -DP antigens. FACS analysis revealed that approximately 40% of the EC population expressed HLA-DR antigen. Quantitation by ELISA demonstrated that maximum expression was observed with 100 U/ml IFN gamma for 4 days. Treatment with IFN gamma also increased class II mRNA levels in all EC cultures tested.
从人脑中分离出的脑血管内皮细胞（EC）并不组成性表达II类主要组织相容性复合体（MHC）抗原。然而，在人γ干扰素（IFNγ）存在的情况下进行孵育，会导致HLA - DR和 - DP抗原的表达。荧光激活细胞分选（FACS）分析显示，约40%的EC群体表达HLA - DR抗原。通过酶联免疫吸附测定（ELISA）进行定量分析表明，在100 U/ml IFNγ作用4天时观察到最大表达量。用IFNγ处理也会增加所有测试的EC培养物中的II类mRNA水平。
