[富集巨大芽孢杆菌中聚-β-羟基丁酸酯（PHB）合成缺陷的突变体]
[Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)].
作者信息
Floccari M E, Tiscornia G, Palmada F, Méndez B S
机构信息
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 1991 Jan-Feb;23(1):26-9.
PMID:1815264
Abstract
Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.
摘要
通过蔗糖梯度离心法，将缺乏聚-β-羟基丁酸酯合成能力的巨大芽孢杆菌的产孢细胞与野生型细胞分离开来。所述条件能够检测出筛选低比例诱变群体中的突变克隆。
Zotero 插件