Hanada Hiroyuki, Fujiwara Yoshiyuki, Sohma Itsuroh, Nakamura Yurika, Takeoka Keiko, Iwatani Yoshinori, Hidaka Yoh
Division of Laboratory for Clinical Investigation, Department of Medical Technology, Osaka University Hospital, Suita.
Rinsho Byori. 2007 Nov;55(11):1002-7.
Peritoneal dissemination is the most frequent recurrent type in advanced gastric cancer. Therefore, the early detection or prediction of peritoneal dissemination is important for the management of patients with advanced gastric cancer. Cytological examination with peritoneal lavage fluids obtained at surgery is widely used for the detection of peritoneal dissemination in gastric cancer. However, the sensitivity of the conventional method is not enough for the prediction of peritoneal recurrence. Recently, molecular diagnosis for detection of cancer cells has been widely studied as the sensitive method for detection of cancer micrometastasis in patients with malignancies including gastric cancer. In this study, novel genetic diagnosis with TRC (transcription reverse transcription concerted reaction) system for detection of carcinoembryonic antigen (CEA) mRNA (Tosoh corporation, Tokyo, Japan) has been introduced for peritoneal lavage diagnosis. We assessed the sensitivity and reproducibility of the test using the diluted gastric cancer cells. Furthermore, the results of TRC with peritoneal lavage fluids obtained from gastric cancer patients has been compared with results of conventional cytology and RT-PCR (reverse transcriptase-polymerase chain reaction).
腹膜播散是进展期胃癌最常见的复发类型。因此，早期检测或预测腹膜播散对于进展期胃癌患者的管理至关重要。手术时获取的腹腔灌洗液细胞学检查广泛用于检测胃癌的腹膜播散。然而，传统方法的敏感性不足以预测腹膜复发。近年来，作为检测包括胃癌在内的恶性肿瘤患者癌症微转移的敏感方法，用于检测癌细胞的分子诊断已得到广泛研究。在本研究中，已引入用于检测癌胚抗原（CEA）mRNA的TRC（转录逆转录协同反应）系统的新型基因诊断方法（日本东京东曹公司）用于腹腔灌洗诊断。我们使用稀释的胃癌细胞评估了该检测方法的敏感性和可重复性。此外，已将从胃癌患者获得的腹腔灌洗液进行TRC检测的结果与传统细胞学和逆转录聚合酶链反应（RT-PCR）的结果进行了比较。
