Rendón Dairo Alonso, Genes Carlos M, Triana Omar
Facultad de Ciencias, Laboratorio de Biofísica, Universidad Nacional de Colombia sede Medellín, Medellin, Colombia.
Biomedica. 2007 Jan;27 Suppl 1:40-9.
Chagas disease is the main cause of cardiomyopathy in endemic regions of Latin America. Alterations in the cardiac mitochondrial energy metabolism caused by Trypanosoma cruzi can be involved in the development of this cardiomyopathy during the course of Chagas disease.
The cellular injury of the rat myocardium was investigated in rats infected with the Colombian Mg8 strain of Trypanosoma cruzi. The activity of mitochondrial ATP synthase was measured to determine the relationship heart damage with the energy metabolism.
Two groups of five rats each were infected with tripomastigotes, with 1 group of 6 rats serving as controls. The course of infection was characterized by parasitological, histopathological and molecular studies. The mitochondrial ATP synthase activity of the myocardium was evaluated in all rats.
Peak parasitaemia (day 26 post infection) and the time of parasite clearance from circulating blood (day 60 post infection) were determined for acute and chronic phase models. The histopathological and molecular results showed that the Colombian Mg8 strain has tropism to the cardiac tissue and causes considerable cellular injury of the myocardium in rats during both phases. Despite the lesions observed in infected rats, no statistical difference in the activity of the mitochondrial ATPsynthase was observed between them and the non-infected rats.
Mitochondrial energy metabolism of the cardiomyocites does not appear to change during cellular injury of rat myocardium associated with infection by the Colombian Mg8 T. cruzi strain.
恰加斯病是拉丁美洲流行地区心肌病的主要病因。克氏锥虫引起的心脏线粒体能量代谢改变可能参与了恰加斯病病程中这种心肌病的发展。
研究感染克氏锥虫哥伦比亚Mg8株的大鼠心肌细胞损伤情况。通过测量线粒体ATP合酶的活性来确定心脏损伤与能量代谢之间的关系。
两组大鼠，每组五只，感染锥鞭毛体，另有一组六只大鼠作为对照。通过寄生虫学、组织病理学和分子学研究来表征感染过程。对所有大鼠的心肌线粒体ATP合酶活性进行评估。
确定了急性和慢性期模型的最高寄生虫血症（感染后第26天）和循环血液中寄生虫清除时间（感染后第60天）。组织病理学和分子学结果表明，哥伦比亚Mg8株对心脏组织具有嗜性，在两个阶段均会导致大鼠心肌细胞受到相当程度的损伤。尽管在感染大鼠中观察到了病变，但感染大鼠与未感染大鼠之间的线粒体ATP合酶活性未观察到统计学差异。
在与哥伦比亚Mg8克氏锥虫株感染相关的大鼠心肌细胞损伤过程中，心肌细胞的线粒体能量代谢似乎没有变化。
