Demers Simon-Pierre, Yoo Jae Gyu, Lian Li, Therrien Jacinthe, Smith Lawrence C
Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
Cloning Stem Cells. 2007 Winter;9(4):512-22. doi: 10.1089/clo.2007.0029.
Despite significant advances achieved through gene targeting in mouse embryonic stem (ES) cells, this technology is presently only available in mice. Because the rat is a species of undeniable importance to biomedical research, attempts at derivation of rat ES cell lines have been ongoing for many years; however, the putative rat ES cell lines that have been reported to date have not yet displayed the ability to contribute in vivo to developing tissues following embryo injection. In contrast to previous studies, we describe herein the successful derivation and characterization of rat ES-like cell lines that not only express markers of undifferentiated cells, alkaline phosphatase (AP) activity and stage-specific embryonic antigen-1 (SSEA-1) cell surface antigen, but also retain expression of Oct4 (also known as Pou5f1) a homeodomain transcription factor and molecular marker of pluripotent cells. Notably, these rat ES-like cells, when injected into blastocysts transferred to pseudopregnant females, can contribute to developing extraembryonic tissues. This report demonstrates for the first time that rat ES-like cells can be derived efficiently, can express a panel of pluripotent cell markers, can be genetically modified in vitro and cryopreserved, and importantly, are capable of contributing to extraembryonic tissues in vivo.
尽管通过对小鼠胚胎干细胞（ES细胞）进行基因靶向取得了重大进展，但该技术目前仅适用于小鼠。由于大鼠是生物医学研究中具有不可否认重要性的物种，多年来一直在尝试建立大鼠ES细胞系；然而，迄今为止报道的假定大鼠ES细胞系尚未显示出在胚胎注射后对发育中的组织进行体内贡献的能力。与先前的研究不同，我们在此描述了大鼠ES样细胞系的成功建立和特性鉴定，这些细胞系不仅表达未分化细胞的标志物、碱性磷酸酶（AP）活性和阶段特异性胚胎抗原-1（SSEA-1）细胞表面抗原，还保留了Oct4（也称为Pou5f1）的表达，Oct4是一种同源结构域转录因子和多能细胞的分子标志物。值得注意的是，这些大鼠ES样细胞在注射到转移至假孕母鼠的囊胚中时，能够对发育中的胚外组织做出贡献。本报告首次证明，大鼠ES样细胞可以高效获得，能够表达一组多能细胞标志物，可以在体外进行基因改造和冷冻保存，并且重要的是，能够在体内对胚外组织做出贡献。
