Font Laura, Miquel Marta, Aragon Carlos M G
Area de Psicobiología, Universitat Jaume I, Castellón 8029AP, Spain.
Physiol Behav. 2008 Mar 18;93(4-5):733-41. doi: 10.1016/j.physbeh.2007.11.026. Epub 2007 Nov 28.
It has been suggested that some of the behavioral effects produced by ethanol are mediated by its first metabolite, acetaldehyde. The present research addressed the hypothesis that catalase-dependent metabolism of ethanol to acetaldehyde in the brain is an important step in the production of ethanol-related affective properties. Firstly, we investigated the contribution of brain catalase in the acquisition of ethanol-induced conditioned place preference (CPP). Secondly, the specificity of the catalase inhibitor 3-amino-1,2,4-triazole (AT) was evaluated with morphine- and cocaine-induced CPP. Finally, to investigate the role of catalase in the process of relapse to ethanol seeking caused by re-exposure to ethanol, after an initial conditioning and extinction, mice were primed with saline and ethanol or AT and ethanol and tested for reinstatement of CPP. Conditioned place preference was blocked in animals treated with AT and ethanol. Morphine and cocaine CPP were unaffected by AT treatment. However, the reinstatement of place preference was not modified by catalase inhibition. Taken together, the results of the present study indicate that the brain catalase-H(2)O(2) system contributes to the acquisition of affective-dependent learning induced by ethanol, and support the involvement of centrally-formed acetaldehyde in the formation of positive affective memories produced by ethanol.
有人提出，乙醇产生的一些行为效应是由其第一种代谢产物乙醛介导的。本研究探讨了以下假设：大脑中过氧化氢酶依赖性的乙醇代谢为乙醛是产生乙醇相关情感特性的重要步骤。首先，我们研究了大脑过氧化氢酶在乙醇诱导的条件性位置偏爱（CPP）形成过程中的作用。其次，用吗啡和可卡因诱导的CPP评估过氧化氢酶抑制剂3-氨基-1,2,4-三唑（AT）的特异性。最后，为了研究过氧化氢酶在再次接触乙醇导致的乙醇寻求复吸过程中的作用，在初始条件化和消退后，用生理盐水和乙醇或AT和乙醇对小鼠进行激发，并测试CPP的恢复情况。用AT和乙醇处理的动物中条件性位置偏爱被阻断。吗啡和可卡因诱导的CPP不受AT处理的影响。然而，位置偏爱的恢复并未因过氧化氢酶抑制而改变。综上所述，本研究结果表明，大脑过氧化氢酶-H₂O₂系统有助于乙醇诱导的情感依赖性学习的形成，并支持中枢形成的乙醛参与乙醇产生的积极情感记忆的形成。
